However, mammalian cell culture is vulnerable to contamination with adventitious agents, including mouse minute virus MMV. The case study presented here demonstrates that MMV is a ubiquitous threat to CHO Chinese hamster ovary cell-based production of biologic drugs and that animal-free media components can be a contamination source. Compounding the risk posed by MMV, the contamination may be "silent," with no impact on cell viability and product titers. Furthermore, contamination may not be detected using in vitro virus assays, and assays based on PCR polymerase chain reaction are required for reliable detection. The development of effective corrective and preventative action CAPA was greatly aided by the identification of the source of the contamination as an animal-free recombinant media additive.
Selection and the Blond Beach Mouse
How Do I Rebrand My Small Business? A Case Study with Mouse Loves Pig - Aeolidia
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Bioethics and Patent Law: The Case of the Oncomouse
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This is a mouse liver single-cell data comprising of 4 different experiments with 3 different protocols. To combine the four liver scRNA-seq datasets, we used a semi-supervised approach by considering the developmental stages as the wanted variation to identify the pseudo-replicates. Note that the cells of the four liver datasets are from different fetal mouse liver developmental stages E9. To identify the pseudo-replicates corresponding to the hepatoblasts, hepatocytes and cholangiocytes, we used three known markers of hepatoblasts and cholangiocytes, Alb, Afp and Epcam, to guide the scMerge algorithm.